![]() The field iris diaphragm, residing in a conjugate plane with the lamp collector lens, is imaged sharply into the same plane as the specimen by the microscope condenser. ![]() This concept is illustrated in Figure 1 with the image of a stained thin section of human muscle tissue superimposed on the iris leaves of the field diaphragm and a focusing reticle in the eyepiece intermediate image plane. When the microscope is properly configured for Köhler illumination, the field diaphragm is imaged in the same conjugate plane as the specimen, and in fact, all of the image-forming conjugate planes are simultaneously imaged into each other and can collectively be observed while examining a specimen in the eyepieces. A new specimen can be selected using the Choose A Specimen pull-down menu. At the smallest opening size setting, only a very minute portion of the specimen is visible in the center of the viewfield. ![]() In order to operate the tutorial, use the Field Diaphragm slider to adjust the opening size and observe how the edges of the diaphragm become visible through the eyepieces. The tutorial initializes with a randomly selected image appearing in the Eyepiece View window and the field diaphragm opening size set to its maximum value (100 percent).
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